Pasteurization Inactivates SARS-CoV-2–Spiked Breast Milk

Holder pasteurization inactivates SARS-CoV-2 in human breast milk. A, SARS-CoV-2 isolates BetaCoV/UKEssen, BetaCoV/Germany/Ulm/01/2020, and BetaCoV/Germany/Ulm/02/2020 were spiked into a medium or individual breast milk samples from donors 1 to 3. B, Isolates BetaCoV/France/IDF0372/2020 and BetaCoV/Netherlands/01 were spiked into medium or milk samples from donors 4 and 5. Isolates were incubated for 30 minutes at room temperature (RT) or 63°C and titrated onto Vero E6 (A) or Caco-2 (B) cells to determine infectious titers. TCID₅₀ was calculated according to the Spearman-Karber method. Data indicate averages and SDs from two (B) or three (A) experiments. LLOQ, lower limit of quantitation (A, 158 TCID₅₀ per mL; B, 176 TCID₅₀ per mL).