INTRODUCTION: As an immunoregulator, Mycobacterium BCG has the potential to be applied in allergic disease such as asthma prevention in clinic. Previous studies showed that neonatal BCG vaccination promoted mouse splenic T helper 1 development.
OBJECTIVE: The objective of this study was to investigate further the impact of BCG vaccination on dendritic cell (DC) development in neonatal mice.
METHODS: Neonatal and adult BALB/C mice were divided into 2 groups: the control group and the BCG-treated group in which BALB/C mice were inoculated with 1 × 105 colony-forming units of BCG intraperitoneally. After 4 weeks, splenic cells were isolated and co-stimulatory molecules and major histocompatibility complex molecules were analyzed by flow cytometry on CD11c-positive cells.
RESULTS: CD11c+CD8α+ and CD11c+CD8α− DCs were found in spleen cells of BALB/C mice. In comparison with the control group, the percentage of CD8α− DCs was significantly decreased (45.00 ± 14.14 vs 67.00 ± 8.27) and that of CD8α+ DCs was strikingly increased (55.00 ± 14.14 vs 33.00 ± 8.27) in BCG-treated neonatal mice. In contrast, the percentage of CD8α− DCs markedly increased from 57% to 70% and that of CD8α+ DCs noticeably decreased from 43% to 30% in adult mice that were vaccinated. BCG vaccination upregulated the expression of co-stimulatory molecules on DC in adult and neonatal mice.
CONCLUSIONS: Our results indicate that development of T cells was induced by BCG vaccination through an effect on DC differentiation and maturation in BALB/C mice, possibly not only by DC phenotype but also by cytokines.
Submitted by Enmei Liu
- Copyright © 2008 by the American Academy of Pediatrics