In seven children with lead poisoning, a thin wet-preparation of erythrocytes was examined under ultraviolet light. In every case red fluorescence was found in 75 to 100% of the erythrocytes. The fluorescence appears to be related to the amount of free protoporphyrin in the erythrocytes. Over 150 preparations of erythrocytes from patients with a variety of other clinical conditions showed 0 to 50% fluorescent erythrocytes. This test is a simple, rapid, reliable method to aid in the diagnosis of lead poisoning in children.
- Received May 4, 1959.
- Accepted June 10, 1959.
- Copyright © 1959 by the American Academy of Pediatrics