OBJECTIVE: Our goal was to explore the influence of duration of status epilepticus on neuronal apoptosis, mitochondrial membrane potential, and cytochrome c release in hippocampus in Wistar rats after status epilepticus (SE).
METHODS: SE that lasted for 30 minutes or 3 hours was induced by intraperitoneal injection with lithium chloride and pilocarpine. Rats were killed at different time points. The apoptosis, mitochondrial membrane potential, and intracellular cytochrome c level were investigated by flow cytometry.
RESULTS: The proportion of apoptotic cells, the decrease of mitochondrial membrane potential, and the release of intracellular cytochrome c significantly changed 30 minutes after 30-minute SE. The peak level was at the 12th hour after SE and 6th hour after SE in apoptosis and the 2 early apoptotic events, respectively. Compared with the same time point after 30-minute SE, the levels of apoptosis and the 2 early apoptotic events after 3-hour SE were much higher. The neuronal apoptosis and the 2 early apoptotic events in hippocampus after SE had a positive correlation with the duration of SE in partial correlation analysis.
CONCLUSIONS: Severe seizures could induce the changes of neuronal apoptosis and the early apoptotic events in hippocampus after SE; the longer the duration of SE, the more serious the change of apoptosis and early apoptotic events were.
Submitted by Li Jiang
- Copyright © 2008 by the American Academy of Pediatrics