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Fig. 2.
Ethidium bromide-stained agarose gel electrophoresis of restriction
endonuclease digestions of VZV polymerase chain reaction products.
Patient's lung biopsy: lanes 1-3. VZV vaccine strain control: lanes
4-6. VZV wild-type strain control: lanes 7-9. Uncut VZV amplification
products: lanes 1, 4, 7. BglI digestion products: lanes
2, 5, 8. PstI digestion products: lanes 3, 6, 9. The
expected 350 bp and 222 bp VZV specific amplification products are seen
for all 3 samples (lanes 1, 4, and 7). The BglI and
PstI digests of the patient's sample are identical to
those of the vaccine control. That is, the 222 bp product is digested
by BglI, resulting in 2 fragments of 137 and 85 bps
(lanes 2 and 5 respectively) and the 350 bp product is not digested by
PstI strain (lanes 3 and 6 respectively). In contrast,
the pattern of the digestions of the wild-type control show that the
222 bp product is not digested with BglI (lane 8) but
that the 350 bp product is digested with PstI, resulting
in 2 fragments of 250 and 100 bp (lane 9).
