PEDIATRICS Vol. 81 No. 1 January 1988, pp. 141-146
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Glycoprotein Filament Removal From Human Milk Fat Globules by Heat Treatment

Wolfgang Buchheim PhD1, Ulrich Welsch MD, PhD1, Gail E. Huston MS1, and Stuart Patton PhD1

1 From the Institute for Chemistry and Physics of the Federal Dairy Research Center, Kiel, and the Anatomy Institute of the University of Munich, Munich, Federal Republic of Germany; and Department of Neurosciences, University of California, San Diego

Freeze-etch electron microscopy was applied to milk fat globules to observe surface details. A remarkable array of filaments, approximately 0.5 µm in length, was seen on human, but not bovine, globules. Heating human globules removed the filaments that were identified as high molecular weight glycoproteins by freezeetch and gel electrophoretic analysis of the heating medium. Extraction of these globule glycoproteins was slight at 60°C for one minute but substantial and tending to plateau at 80°C for one minute. Such heat-induced alterations of the milk fat globule surface provide an alternative or additional explanation to milk lipase inactivation as the cause of reduced milk fat absorption from heated milk by the preterm infant.

Key Words: milk • glycoprotein • membrane • fat absorption • premature infant

Submitted on December 11, 1987
Accepted on April 13, 1987




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