PEDIATRICS Vol. 8 No. 1 July 1951, pp. 82-84
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SOURCE OF ERROR IN TEST FOR STOOL TRYPSIN

PAUL C. GAFFNEY M.D.1

1 The Department of Pediatrics, University of Pittsburgh School of Medicine and the Children's Hospital of Pittsburgh, Pittsburgh.

A source of error in the gelatin film test for stool trypsin is reported. Identical results are obtained with Kodak and DuPont film when incubation at room temperature for two hours is used.

The gelatin on DuPont film incubated at 37° C. for one hour is digested by stools from infants with mucoviscidosis and also by distilled water as well as by stools from healthy infants. Stools from some infants with mucoviscidosis will digest the gelatin on Kodak film when incubated at 37° C. for two hours.

Kodak film should be used if the preparations are to be incubated at 37° C. for one hour, as recommended in the original description of the technic.

Submitted on May 8, 1950