PEDIATRICS Vol. 76 No. 6 December 1985, pp. 965-969
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Changes in Cell Proliferation Kinetics in the Mouse Cerebellum After Total Asphyxia

Hiroshi Yoshioka MD1, Masahiro Mino MD1, Yuji Morikawa MD1, Yasuo Kasubuchi MD1, and Tomoichi Kusunoki MD1

1 From the Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto, Japan

This study was undertaken to investigate the effects of neonatal asphyxia on brain development, with special reference to the kinetics of neuronal proliferation by using autoradiography. For 30 minutes, two-day-old suckling mice, Jcl:ICR strain, were put into a chamber which was constantly flushed with 100% CO2 gas. After the exposure to asphyxia, 29% of the mice survived. Cell cycle studies were carried out at two days and at seven days on the external matrix cells, the precursor of the granule cells, at the external granular layer of the cerebellum from CO2-exposed and control mice by 3H-thymidine autoradiography. At two days the generation time of the control mice was about 15 hours, whereas that of the asphyxiated mice was about 17 hours. The prolongation of the generation time in the asphyxiated mice was caused mainly by a delay in the G2 phase. This prolongation was apparent for about five days and there-after growth caught up. These results suggest that neonatal asphyxia has an adverse effect on cerebellar neuronal proliferation that may revert to normal spontaneously in older animals.

Key Words: neonate • asphyxia • cell proliferation • cerebellum • autoradiography

Submitted on October 22, 1984
Accepted on January 18, 1985




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