PEDIATRICS Vol. 64 No. 5 November 1979, pp. 632-634
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Countercurrent Immunoelectrophoresis in Group B Streptococcal Disease

Barbara W. Stechenberg MD1, Richard L. Schreiner MD1, Susan M. Grass BS1, and Penelope G. Shackelford MD1

1 Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St Louis, and Indiana University School of Medicine, Indianapolis

Countercurrent immunoelectrophoresis was used for the detection of group- and type-specific antigens in the body fluids of 61 infants from St. Louis and Indiana with group B streptococcal infections. Urine concentrated using an Amicon filter yielded the highest percentage of positive results; 81% were positive in the St Louis group. When three body fluids (urine, CSF, and blood) were available, at least one was positive for group B streptococcus in 95% of the cases. This study demonstrates the applicability of this test in a tertiary care facility (St Louis) and in smaller hospitals (Indiana) with access to central laboratory.

Submitted on February 5, 1979
Accepted on April 9, 1979


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