PEDIATRICS Vol. 35 No. 2 February 1965, pp. 361
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Extraction Methods for Bilirubin Assay; Bilirubin Monoglucuronide in Neonatal Hyperbilirubinemia

DEREK WATSON D.Sc., F.R.I.C.1

1 Head, Division of Biochemistry, Royal Women's Hospital, Melbourne, Swanston St. Carlton, N.3 Victoria, Australia

As Tsvet, the originator of adsorption chromatography, remarked in 1920: "Although all scientific progress is progress in technic, technic is not uncommonly the weakest part of scientific investigation." Certainly, the experiences of Mr. Ibbott and Dr. O'Brien (Pediatrics, 34: 418, 1964) provide a good illustration of this, and I entirely agree with their conclusions that solvent partition methods for measuring neonatal serum bile pigments are unsatisfactory and that earlier "conclusions on the possible significance of the monoglucuronide are invalid."