PEDIATRICS Vol. 28 No. 4 October 1961, pp. 662-669
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MICROMETHOD FOR DETERMINATION OF CONCENTRATION OF ANTIBIOTICS IN SERUM FOR APPLICATION IN CLINICAL PEDIATRICS

R. Fujii M.D.1, M. Grossman M.D.1, and W. Ticknor B.A.1

1 Department of Pediatrics, San Francisco General Hospital and University of California Medical Center

A micromethod is described for determination of concentration of antibiotics, utilizing agar diffusion, beta-hemolytic streptococci as the test organism, and blood hemolysis as an indicator. The method can be performed with 0.1 ml of serum and does not require rigorous sterile technique for the filling of capillary tubes or for the collection of blood. Duplicate determinations of 61 serum specimens were made, comparing the concentrations of erythromycin propionate lauryl sulfate obtained by the standard Rammelkamp twofold tube dilution method with those obtained by our micromethod. Only 1 of the 61 specimens analyzed showed more than one twofold dilution difference between the two methods. This micromethod appears to be suitable for the determination of actual concentrations of any antibiotic that is effective against beta-hemolytic streptococci, with only minor variations in the performance of the test. It might also be modified for antibiotics that are not effective against beta-hemolytic streptococci by using another test organism and, for example, a dye indicator.