1 Department of Pediatrics, Western Reserve University School of Medicine, and Department of Pathology at Cleveland City Hospital
The nephrotic syndrome produced in rats by administration of the aminonucleoside of Puromycin® appears after a latent period which can be shortened or lengthened by increasing or reducing the daily dose. Young rats which survive the initial course lose their proteinuria, but some relapse after 3 to 4 months. Upon re-administration of the nucleoside a chronic disease can be induced which causes death in 3 to 8 months from renal failure. Older rats given a single intravenous injection develop a chronic disease with a natural history the details of which are still not known.
It is unlikely that the pathogenesis is based on an antigen-antibody reaction, since ACTH, cortisone and inhibition of antibody formation by x-radiation fail to prevent the disease.
The nucleoside fails to produce a similar disease in dogs, rabbits, guinea pigs, and mice. Both 3-amino-3-deoxy-D-ribose, and and 6-dimethyl aminopurine, which in combination form the nucleoside, are inactive in producing renal disease individually. It is possible that dogs, rabbits, guinea pigs and mice are able to break down this compound while rats cannot.
Submitted on October 2, 1957