PEDIATRICS Vol. 21 No. 1 January 1958, pp. 7
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Clinical and Laboratory Studies of Mumps. I. Laboratory Diagnosis by Tissue-Culture Technics

The use of tissue-culture technics in primary isolation of virus from patients with mumps and its complications is reported. In addition to recovery from saliva, the virus was repeatedly isolated from cerebrospinal fluid and urine. From the latter source, virus was recovered as long as 13 days from onset of illness. The virus was propagated in both monkey-kidney and HeLa-cell cultures.