Published online April 21, 2008
PEDIATRICS Vol. 121 No. 5 May 2008, pp. e1348-e1351 (doi:10.1542/peds.2007-2215)

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ARTICLE

Erythroid Apoptosis in Idiopathic Neonatal Jaundice

Hasan Özkan, MD^a, Hale Ören, MD^b, Mansur Tatl, MD^a, Halil Ate, MSc^c, Abdullah Kumral, MD^a, Nuray Duman, MD^a

^a Departments of Pediatric Neonatology
^b Pediatric Hematology
^c Hematology Laboratory, Dokuz Eylül University Faculty of Medicine, Izmir, Turkey

OBJECTIVES. The objectives of this study were to evaluate the contribution of erythroid apoptosis to neonatal idiopathic pathologic jaundice and to determine whether a measurement of the erythroid apoptosis value at birth could predict the development of hyperbilirubinemia during the first 15 days of life.

PATIENTS AND METHODS. Three groups were defined: group 1 (n = 101), healthy newborns whose erythroid apoptosis value and serum total bilirubin levels were detected from birth to day 15; group 2 (n = 24), newborns who were hospitalized for jaundice (serum total bilirubin level: >12.9 mg/dL) without any identifiable pathologic cause; and group 3 (control group, n = 24), healthy newborns whose serum total bilirubin levels were 12.9 mg/dL. Erythroid apoptosis value was assessed by flow cytometry using an annexin-V fluorescein isothiocyanate kit.

RESULTS. In group 1, there was no correlation between the erythroid apoptosis value and serum total bilirubin levels obtained at birth and at the fourth and 15th days of life; the erythrocyte apoptosis value obtained at birth was not significantly different between the neonates whose serum total bilirubin levels were >12.9 and 12.9 mg/dL and who had prolonged and nonprolonged jaundice during follow-up. The erythroid apoptosis value differed significantly between the newborns in groups 2 and 3. There was no significant correlation between the erythroid apoptosis value and serum total bilirubin levels of the infants in groups 2 and 3.

CONCLUSIONS. The erythroid apoptosis value obtained at birth could not predict the development of hyperbilirubinemia in neonates, but it was increased significantly in jaundiced neonates whose serum total bilirubin levels were >12.9 mg/dL. In these infants, increase in the erythroid apoptosis value may be a result of the toxic effect of bilirubin or of a protective mechanism of neonates to increase heme turnover and bilirubin production to diminish oxidative stress.

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Key Words: apoptosis • erythrocyte • jaundice • hyperbilirubinemia • newborn

Abbreviations: STB—serum total bilirubin

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Accepted Nov 8, 2007.

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