This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services E-mail this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to My File Cabinet Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nupponen, I. Articles by Repo, H. Search for Related Content PubMed PubMed Citation Articles by Nupponen, I. Articles by Repo, H. Related Collections Premature & Newborn Social Bookmarking                         What's this?

PEDIATRICS Vol. 110 No. 1 July 2002, pp. 36-41

Neutrophil Activation in Preterm Infants Who Have Respiratory Distress Syndrome

Irmeli Nupponen, MD^*,, Eero Pesonen, MD, PhD, Sture Andersson, MD, PhD^,||, Aila Mäkelä, MD^*,, Riikka Turunen, BM^*,, Hannu Kautiainen, BA^¶ and Heikki Repo, MD, PhD^*,#

^* Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
Department of Anesthesiology, University of Helsinki, Helsinki, Finland
Hospital for Children and Adolescents, Helsinki, Finland
^|| Department of Obstetrics and Gynecology, University of Helsinki, Helsinki, Finland
^¶ Rheumatism Foundation Hospital, Heinola, Finland
^# Department of Medicine, Division of Infectious Diseases, University of Helsinki, Helsinki, Finland

Objective. To study neutrophil activation in circulation as a sign of systemic inflammation in preterm infants with respiratory distress syndrome.

Methods. The study comprised very low birth weight preterm infants who had respiratory distress syndrome and required intubation and mechanical ventilation (n = 51), 1-day-old preterm infants who had no need for mechanical ventilation (n = 12), term infants (n = 47), and adult volunteers (n = 25). Neutrophil surface expression of CD11b was quantified with flow cytometry.

Results. In preterm infants with respiratory distress syndrome, neutrophil CD11b expression during the first day of life was higher than in cord blood (mean: 165 relative fluorescence units [RFU] [standard deviation [SD]: 53], n = 29 vs 83 RFU [SD: 21], n = 11; 95% confidence interval [CI] for difference: 59–106) or in preterm infants without mechanical ventilation (106 RFU [SD: 33], n = 12; 95% CI for difference: 17–90). CD11b expression decreased by age of 10 days. CD11b expression was lower in preterm cord than in term cord blood (95% CI for difference: 5–53). However, in preterm infants with respiratory distress syndrome aged 2 to 5 days, it was higher than in term infants of that age.

Conclusions. The observations demonstrate an early transient postnatal neutrophil activation indicative of systemic inflammation that may contribute to the tissue injury in preterm infants with respiratory distress syndrome.

Key Words: CD11b/CD18 • C-reactive protein • neutrophil activation • preterm infant • respiratory distress syndrome • systemic inflammation

Abbreviations: ARDS, adult respiratory distress syndrome • FMLP, formyl-methionyl-leucyl-phenylalanine • RDS, respiratory distress syndrome • BPD, bronchopulmonary dysplasia • FIO₂, fraction of inspired oxygen • SD, standard deviation • FACS, fluorescence-activated cell sorter • RFU, relative fluorescence unit • ANOVA, analysis of variance • CI, confidence interval

---

Received for publication Jun 26, 2001; Accepted Dec 13, 2001.

CiteULike    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				F.-C. Cheah, J. J. Pillow, B. W. Kramer, G. R. Polglase, I. Nitsos, J. P. Newnham, A. H. Jobe, and S. G. Kallapur Airway inflammatory cell responses to intra-amniotic lipopolysaccharide in a sheep model of chorioamnionitis Am J Physiol Lung Cell Mol Physiol, March 1, 2009; 296(3): L384 - L393. [Abstract] [Full Text] [PDF]

				P. Henneke and R. Berner Interaction of neonatal phagocytes with group B streptococcus: recognition and response. Infect. Immun., June 1, 2006; 74(6): 3085 - 3095. [Full Text] [PDF]

				R. Turunen, I. Nupponen, S. Siitonen, H. Repo, and S. Andersson Onset of Mechanical Ventilation Is Associated With Rapid Activation of Circulating Phagocytes in Preterm Infants Pediatrics, February 1, 2006; 117(2): 448 - 454. [Abstract] [Full Text] [PDF]