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PEDIATRICS Vol. 102 No. 1 Supplement July 1998, pp. 236-237

COMMENTARY:
A Simple Phenylalanine Method for Detecting Phenylketonuria in Large Populations of Newborn Infants, by Robert Guthrie and Ada Susi, Pediatrics, 1963;32:318-343

Received Mar 19, 1998; accepted Mar 19, 1998.

Comments by Charles R. Scriver

From the DeBelle Laboratory for Biochemical Genetics, McGill University-Montreal Children's Hospital Research Institute, Montreal, Quebec H3H 1P3 Canada

The article describes a "microbial inhibition assay" for rapid and economical measurement of phenylalanine levels in whole blood. Capillary blood, from a heel prick, is collected from the newborn infant onto Schleicher and Schuell no. 903 filter paper; a disk of the sample is then transferred to an Agar plate containing a heavy inoculum of Bacillus subtilis ATCC 6051; an inhibitor of bacterial growth (beta -2-thienylalanine) is counteracted by any significant excess of phenylalanine in the blood sample; and semiquantitative positive tests (hyperphenylalaninemia) are recorded by size of the bacterial growth zone in the Agar around the filter paper disc. The method permits mass screening for hyperphenylalaninemia.


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