Click here to download a slide of this image in PowerPoint format. You do NOT need to request permission if you are reproducing or paraphrasing a table or figure to make slides or handouts for an educational lecture or discussion that is under the auspices of a not-for-profit educational organization. Otherwise, contact permissions{at}aap.org for additional information or to make a request. [View Larger Version of this Image (304K JPEG file)]

Fig. 2.   Ethidium bromide-stained agarose gel electrophoresis of restriction endonuclease digestions of VZV polymerase chain reaction products. Patient's lung biopsy: lanes 1-3. VZV vaccine strain control: lanes 4-6. VZV wild-type strain control: lanes 7-9. Uncut VZV amplification products: lanes 1, 4, 7. BglI digestion products: lanes 2, 5, 8. PstI digestion products: lanes 3, 6, 9. The expected 350 bp and 222 bp VZV specific amplification products are seen for all 3 samples (lanes 1, 4, and 7). The BglI and PstI digests of the patient's sample are identical to those of the vaccine control. That is, the 222 bp product is digested by BglI, resulting in 2 fragments of 137 and 85 bps (lanes 2 and 5 respectively) and the 350 bp product is not digested by PstI strain (lanes 3 and 6 respectively). In contrast, the pattern of the digestions of the wild-type control show that the 222 bp product is not digested with BglI (lane 8) but that the 350 bp product is digested with PstI, resulting in 2 fragments of 250 and 100 bp (lane 9).